Gabriel Jesus tamely missed a 43rd-minute penalty - an untimely reminder of his side's frailties from the spot given Riyad Mahrez blazed over during last season's goalless draw at Liverpool.
Guardiola has since tried to backtrack on his comments, but Mane has come out fighting ahead Sunday's crunch game between the two sides. "I do not dive", said Mane.
Premier League leaders Liverpool host reigning champions Manchester City at Anfield on Sunday.
Klopp launched a defence of his team and Mane after Guardiola appeared to suggest the Senegal global was prone to diving.
"The only thing I can say I will be ready for the team, to give everything possible, and to help my team".
Asked about this at a press conference in Italy ahead of Wednesday's Champions League clash against Atalanta, Guardiola said: "No comment on that". He did previous year something similar, where he was out for a game and then I think a couple of days later he was back on the pitch. Why not? But what coach Jurgen [Klopp] said is correct. I can say Sadio is not a diver.
"For me, he's the best manager in the world, and we have the chance to win against his team which is very hard but possible which is enough for me". If I get a penalty, yeah it's a penalty.
"I've never seen him go into a big match and play defensively", said John.
A similar incident might well have occurred in his plush city centre apartment before morning training and Manchester City's subsequent flight to Milan.
However, Guardiola has now gone back on his comments, stating that Liverpool's last-minute goal against Leicester last month was indeed lawful, after the forward was fouled inside the box and the resulting penalty was scored. Even with that for Jurgen it [against Leicester] is a penalty, for the referee it was a penalty, for the VAR it was a penalty.