Biles won her 24th and 25th World Championship medals on Sunday, breaking the record for total medals set in the 1990s by Vitaly Scherbo of Belarus.
That ties the record for the most world medals for any gymnast.
The only blot on her near-perfect record in south-west Germany came in Saturday's uneven bars final when she finished fifth, but both Biles and her coach Laurent Landi were delighted with her week.
Biles has won five events at these world championships after team gold Tuesday, individual all-around gold Thursday and vault gold Saturday.
Biles won the vault by more than half a point, averaging 15.399 for her two attempts.
Biles later missed the chance of a second medal, finishing fifth in the uneven bars final behind victor Nina Derwael of Belgium, who retained the title she won in Doha a year ago.
As another title-winning score was announced in the arena, Biles punched the air in jubilation before joining celebrations with the USA team.
Biles scored 14.7, finishing by sticking the landing on a dismount with two flips and two twists.
A year after becoming the Philippines' first gymnast to win a medal, Carlos Yulo took victory in the men's floor exercise. As Nellie Biles told her daughter via Twitter at the outset of this competition: "Be the Best Simone". "It definitely didn't go as well as the training past year, but going into the gym we'll focus a lot more on that vault", she said.
"I never think of medal counts or anything", she said after winning her record-tying 23rd and record-extending 17th gold in the vault final on Saturday, the penultimate day of worlds.
"I'm excited with the performances I put out today, obviously no medal on the bars, but there was (only) a slim chance I was going to (get one)", admitted Biles.
Britain's Olympic champion Max Whitlock won his third world title in pommel horse, scoring 15.5 with a near-flawless routine.
This is the first time she has finished a world championships with five golds, but she has said she is "99 percent" sure Stuttgart will be her last. "I think of my performances and then..."
Last year's bronze medallist, Yulo performed the most hard routine of any of the eight finalists to score 15.3 and beat Israel's Artem Dolgopyat by one-tenth of a point.
Whitlock scored 15.5 with a high-difficulty routine in the pommel horse.
The 26-year-old added to his 2015 and 2017 titles with a winning margin of only 0.067 points ahead of silver medallist Lee Chih-Kai of Taiwan. And her work is hardly done in what she has said is nearly certainly her final world championships.