At current value, this means her stake is worth more than $35 (£37) billion, according to Forbes. She also gave up her interests in the Washington Post, owned by Jeff Bezos independently from Amazon, and his space exploration company Blue Origin.
Amazon founder Jeff Bezos will keep 75% of the shares in the ecommerce giant that were jointly held with his wife, MacKenzie - and he will have full voting control of the couple's shares - after the two finalized their divorced.
The Bezos owned 78.8 million shares, or about 16% of the company.
The couple's announcement to split came just before The National Enquirer published a story saying Mr Bezos was having an affair with a former TV host.
"Grateful to have finished the process of dissolving my marriage with Jeff", MacKenzie Bezos said on Twitter, in the first and only tweet from an account that was created this month.
But some outlets seem to have gotten things backwards, reporting that MacKenzie Bezos is "receiving" stock or that Jeff Bezos is "giving it up", as if he's doing it out of the kindness of his heart. Jeff Bezos founded Amazon in their Seattle garage in 1994 and turned it into a colossus that dominates online retail.
Jeff Bezos and his wife MacKenzie have settled their divorce - and the richest man in the world got off lightly. Amazon shares dipped less than 1 per cent at 1:38 p.m.in NY. "I'm grateful for her support and for her kindness in this process".
Following the terms being disclosed, Bezos, who remains Amazon's chief executive and largest shareholder, took to Twitter to express his feelings on the end of this chapter.
MacKenzie Bezos, a novelist, said in a tweet Thursday that she was "excited" about her future plans, but didn't say what they were.
That investigation eventually implicated Sanchez's brother, Michael, who has always been a supporter of Donald Trump.