By Tuesday, the Iranian Central Bank said new measures on foreign currencies will take effect.
"You can not expect to talk to a person, after you stab him and leaving the knife in his body", Rouhani, speaking in Persian, told IRIB state televison.
In May, President Donald Trump withdrew the United States from an worldwide 2015 deal to control Iran's nuclear ambitions. USA officials last week said Iran carried out a similar exercise, though Tehran did not immediately acknowledge it. China's refusal to comply with USA sanctions, likewise, is a sign that Iran won't be totally cutoff from world markets.
Officials Monday also repeated the words of Secretary of State Mike Pompeo: That the U.S.is not seeking regime change, but a change in the regime's behavior. "They don't understand the meaning of this statement, because it makes no sense for Iranian oil not to be exported, while regional oil exports continue", he said.
It will be an additional 90 days before the rest of the sanctions "snap back" into place in early November.
So-called snapback sanctions, due to come into force early on Tuesday, target Iranian purchases of US dollars, metals trading, coal, industrial software and its auto sector.
The EU announced that its "blocking statute" - aimed at "sustaining trade and economic relations between the EU and Iran" in the face of renewed USA sanctions - will enter into force on the same day as the restored sanctions.
"We are determined to protect European economic operators engaged in legitimate business with Iran", the statement added.
"I have a suspicion that even if it doesn't come directly, I can easily see in six months the Iranians turning to the Russians and letting the Russians be their channel", to negotiate with Trump, said Ross."Given the Trump-Putin relationship, we can see Russia coming and offering something, opening up a negotiation".
Ahmad Majidyar, director of IranObserved Project at the Middle East Institute, said even before the sanctions took effect, Iran's vulnerable economy had already been undermined by the USA withdrawal from the nuclear deal. But they denied security forces were involved, Iranian news agencies reported.
"It also forbids EU persons from complying with those sanctions, unless exceptionally authorized to do so by the Commission in case non-compliance seriously damages their interests or the interests of the Union".
Bolton said that the sanctions show how strongly the administration feels that the Iranian nuclear weapons program needs to end.
Trump repeatedly has tweeted that he is willing to talk directly to Iranian officials without preconditions.
Rouhani: What Trump is doing is against Iran and the Iranian people.
They have pledged to abide by their commitments under the deal.
By dealing through Putin and not Trump, Iran can save face while it deals with the defeat dealt to it by Trump's withdrawal from the deal, and its own economic incompetence. When Iran's military has lashed out or tested the U.S. in Syria, the United States has beaten them back emphatically, as has Israel.
Still, Europe's top diplomats vowed on Monday to work together to preserve the 2015 deal.
"We deeply regret the reimposition of sanctions by the USA", the bloc said in a joint statement with the foreign ministers of France, Germany and Britain.
"We deeply regret the re-imposition of sanctions by the USA, due to the latter's withdrawal from the Joint Comprehensive Plan of Action (JCPOA)", the statement issued in Brussels said.
Trump had denounced the deal reached under his White House predecessor, Barack Obama, as one-sided in Iran's favor.
Rouhani expressed concern that Trump has backed out of previous dialogue and negotiations with Iran.
Already, the amount of Iranian crude imports to Korea dwindled by 18-and-a-half percent during the first half of this year from the same period last year to 3-point-3-billion dollars.
She said the collapse in the value of the rial was not driven by purely economic factors but instead by people rushing to buy gold or hard currency as a safe haven for their savings because they do not trust the government to improve the situation.