Rowley and Sturgess were poisoned by the nerve agent just months after the former Russian spy Sergei Skripal and his daughter, Yulia, were exposed to Novichok.
"Charlie has been through an appalling experience most of us could never imagine", she said.
Detectives believe a team of Russians assassins were sent to the United Kingdom to murder the former Kremlin double agent back in March and are hunting up to six suspects.
Britain blamed Russia for the poisoning of Skripal, a former military intelligence colonel who was jailed for betraying Russian agents to Britain's MI6 foreign intelligence service.
Russian Federation has repeatedly denied any involvement in the attack and has put forward multiple theories as to who may have been behind it.
Salisbury District Hospital director of nursing Lorna Wilkinson confirmed Mr Rowley had been discharged.
'We continue to think of Charlie and of Dawn's family, and support them as they go through the hard process of coming to terms with her death'.
The second round of nerve agent poisonings put the towns of Amesbury and Salisbury, where Sturgess lived, on edge as more than 100 counterterrorism officers searched for the source of the Novichok.
A source who has been briefed on the progress of the investigation said: "They want to get out of Charlie the best account of where he was and what happened so they can get to the bottom of this matter".
London Metropolitan Police, who are leading the investigation into the Novichok poisoning, said the nerve agent was found in a "small bottle" in Rowley's Amesbury, Wiltshire, home.
Public Health England has said Mr Rowley, 45, poses no risk to the community, but said the public should not pick up random items off the street.
Sturgess and Rowley were admitted to Salisbury District Hospital, the same hospital where the Skripals were treated.
PA said investigators believe Sturgess was exposed to at least 10 times the amount of nerve agent as the Skripals came into contact with.
Rowley's brother Matthew told the BBC that the 45-year-old had told him the Novichok was contained in a perfume bottle.
The Organisation for the Prohibition of Chemical Weapons (OPCW) will analyse the substance before reporting back its conclusions.
On Thursday, a coroner's inquest was opened and adjourned for mother-of-three Ms Sturgess.